mouse α human igg1 fc  (SouthernBiotech)

Journal: Vaccines

Article Title: Dynamics of Serum-Neutralizing Antibody Responses in Vaccinees through Multiple Doses of the BNT162b2 Vaccine.

doi: 10.3390/vaccines11111720

Figure Lengend Snippet: Figure 1. Vaccination induces durable SARS-CoV-2 spike RBD-specific serum IgG and IgA responses. The time course of BNT162b2 vaccine delivery and serum collection is shown (A). Receptor-binding

Article Snippet: For IgG subclass-specific assays, binding events were detected using HRP-labeled mouse α-human IgG1 Fc, IgG2 Fc, IgG3 hinge, and IgG4 Fc secondary antibodies (1:2000, Southern Biotech, Birmingham, AL, USA).

Techniques: Binding Assay

Journal: Vaccines

Article Title: Dynamics of Serum-Neutralizing Antibody Responses in Vaccinees through Multiple Doses of the BNT162b2 Vaccine.

doi: 10.3390/vaccines11111720

Figure Lengend Snippet: Figure 2. Booster vaccinations induce IgG4-switched Ag-specific responses in serum. RBD-reactive (A) and spike-reactive (B) IgG subclass responses (Wuhan-Hu-1) were measured by ELISA as de- scribed in Methods. Statistical significance was determined as in Figure 1. For clarity, statistical comparisons only for post-vax time points 2A and later are shown. ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, not significant.

Article Snippet: For IgG subclass-specific assays, binding events were detected using HRP-labeled mouse α-human IgG1 Fc, IgG2 Fc, IgG3 hinge, and IgG4 Fc secondary antibodies (1:2000, Southern Biotech, Birmingham, AL, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Vaccines

Article Title: Dynamics of Serum-Neutralizing Antibody Responses in Vaccinees through Multiple Doses of the BNT162b2 Vaccine.

doi: 10.3390/vaccines11111720

Figure Lengend Snippet: Figure 3. Vaccination elicits potent but transient neutralizing antibody responses in serum. Neutral- izing antibody responses were evaluated in SARS-CoV-2 spike pseudotyped virus inhibition assays (A) as described in Methods. Statistical significance was determined using two-way ANOVA with Tukey’s multiple comparisons of mean NT50 values at each sampling point: *** p = 0.003, **** p < 0.0001. Correlations between serum NT50 values and RBD-reactive IgG or IgA levels were determined at post-vax time points 2A and 2B (B,C) and post-boosting at time points 3A and 3B (D,E); Pearson coefficients of correlation (r) are listed on each graph. ns, not significant.

Article Snippet: For IgG subclass-specific assays, binding events were detected using HRP-labeled mouse α-human IgG1 Fc, IgG2 Fc, IgG3 hinge, and IgG4 Fc secondary antibodies (1:2000, Southern Biotech, Birmingham, AL, USA).

Techniques: Virus, Inhibition, Sampling

Journal: Vaccines

Article Title: Dynamics of Serum-Neutralizing Antibody Responses in Vaccinees through Multiple Doses of the BNT162b2 Vaccine.

doi: 10.3390/vaccines11111720

Figure Lengend Snippet: Figure 4. BNT162b2-induced serum IgG responses show impaired reactivity against variant SARS- CoV-2 spike RBD proteins that is improved by vaccine boosting. IgG levels against variant SARS- CoV-2 spike RBD proteins were measured by ELISA at time point 2A (A,C) and post-boosting at time point 3A (B,C) as described in Methods. Statistical significance was determined as in Figure 3. **** p < 0.0001. Reactivity for each variant at time points 2A and 3A was compared using paired t-tests. *** p = 0.0003, **** p < 0.0001.

Article Snippet: For IgG subclass-specific assays, binding events were detected using HRP-labeled mouse α-human IgG1 Fc, IgG2 Fc, IgG3 hinge, and IgG4 Fc secondary antibodies (1:2000, Southern Biotech, Birmingham, AL, USA).

Techniques: Variant Assay, Enzyme-linked Immunosorbent Assay